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CD133 (Prominin-1), clone: 13A4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1331-80

Additional Details : Weight : 0.01000kg

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Product Code. 15267207

  • 766.00 SEK / 25µg


EU Stock 1
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Description

Description

Description: The 13A4 monoclonal antibody recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural and endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown. The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen but has been reported to work in canine/dog.Applications Reported: The 13A4 antibody has been reported for use in flow cytometric analysis (∽0.25-1 μg/million cells), immunoprecipitation (∽10-25 μg/mL), immunoblotting (WB) (∽1-5 μg/mL), and immunohistochemical staining (∽1-10 μg/mL).Applications Tested: The 13A4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD133 (Prominin-1)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
O54990
AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Murine
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot, Western Blot
13A4
Unconjugated
Prom1
Liquid
PROM1
IgG1, kappa, kappa
25μg
Store at 2-8°C.
Primary
19126, 488816
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