CD135 (Flt3), clone: A2F10, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1351-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.Applications Reported: The A2F10 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.Applications Tested: The A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (Fluorochrome-conjugated A2F10 is recommended for use in flow cytometry.).Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|RP11-153M24.3, CD135, FLK2, STK1|
|Flow Cytometry, Immunoprecipitation|
|IgG2a, kappa, kappa|
For Research Use Only.