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CD135 (Flt3) Monoclonal Antibody (A2F10), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

1220.00 SEK - 2705.00 SEK

Specifications

Antigen CD135 (Flt3)
Clone A2F10
Concentration 0.5 mg/mL
Applications Flow Cytometry, Immunoprecipitation
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15247217
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Affymetrix eBioscience
14-1351-82
100 μg
1220.00 SEK
100µg
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15257217
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Affymetrix eBioscience
14-1351-85
500 μg
2705.00 SEK
500µg
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Estimated Shipment: 07-05-2024
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Description

Description

Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively. Applications Reported: The A2F10 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. Applications Tested: The A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (Fluorochrome-conjugated A2F10 is recommended for use in flow cytometry.). Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Specifications

Specifications

CD135 (Flt3)
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
B230315G04; CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK2; Flk-2; FLT3; Flt-3; fms related tyrosine kinase 3; FMS-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Ly72; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; stem cell tyrosine kinase 1; STK1; STK-1; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; wmfl
FLT3
Primary
4° C
FLT3
A2F10
Flow Cytometry, Immunoprecipitation
Unconjugated
Rat
Mouse
Q00342
14255
IgG2a κ
Affinity chromatography
Antibody
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