CD137 Ligand (4-1BB Ligand), PerCP-eFluor™ 710, clone: TKS-1, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5901-82
Additional Details : Weight : 0.09500kg
DescriptionDescription: The TKS-1 monoclonal antibody reacts with mouse 4-1BB Ligand (4-1BBL), a type II transmembrane protein and a member of the tumor necrosis factor superfamily. 4-1BBL is expressed by activated antigen presenting cells including B cells and macrophages. Interaction of 4-1BBL with 4-1BB (CDw137) is reported to deliver a costimulatory signal important in the T-APC interaction.Applications Reported: This TKS-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TKS-1 antibody has been tested by flow cytometric analysis of stimulated mouse spleen cells. This can be used at less than or equal to 0.5μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL cell sample + 100μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|CD137 Ligand (4-1BB Ligand)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|TNFSF9, CDw137L, CD137L, 4-1BBL, 41BBL|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.