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Invitrogen™ CD14 Monoclonal Antibody (61D3), APC, eBioscience™, Invitrogen™
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Produktkod. 15517886
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Produktkod. 15517886 Leverantör Invitrogen™ Leverantörsnummer 17014942

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Beskrivning

Mouse Monoclonal Antibody

Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14. Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.
TRUSTED_SUSTAINABILITY

Specifikationer

Antigen CD14
Användningsområden Flow Cytometry
Klassificering Monoclonal
Klona 61D3
Koncentration 5 μL/Test
Konjugera APC
Formulering PBS with BSA and 0.09% sodium azide; pH 7.2
Gen Cd14
Genaccessionsnr. P08571
Gene Alias CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
Gensymboler Cd14
Värd art Mouse
Reningsmetod Affinity chromatography
Kvantitet 100 Tests
Regulatorisk status RUO
Primär eller sekundär Primary
Gen-ID (Entrez) 929
Målarter Human
Innehåll och lagring 4°C, store in dark, DO NOT FREEZE!
Produkttyp Antibody
Form Liquid
Isotyp IgG1 κ
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For Research Use Only.

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