CD140a (PDGFRA), clone: APA5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1401-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.Applications Reported: The APA5 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified APA5 in functional assays.).Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of the NIH/3T3 cell line. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|PDGF Receptor alpha, MGC74795, PDGFR2, RHEPDGFRA, CD140 antigen-like family member A, CD140a antigen, PDGF-R-alpha, PDGFRA/BCR fusion, pdgfr-a|
|Flow CytometryþImmunohistochemistry (Frozen)þWestern Blot|
|IgG2a, kappa, kappa|
For Research Use Only.