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CD140b (PDGFRB), clone: APB5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1402-81

Additional Details : Weight : 0.01000kg

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Product Code. 15247227

  • POA / 50µg

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Description

Description

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.Applications Reported: The APB5 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified APB5, cat. 16-1402, in functional assays.).Applications Tested: The APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD140b (PDGFRB)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P05622
PDGF Receptor beta, JTK12, PDGFR, PDGFR1, CD140 antigen-like family member B, PDGF-R-beta, beta-type platelet-derived growth factor receptor
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Immunohistochemistry (Frozen)
APB5
Unconjugated
Pdgfrb
Liquid
Pdgfrb
IgG2a, kappa, kappa
50μg
4° C
Primary
18596
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