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CD140b (PDGFRB) Monoclonal Antibody (APB5), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

550.00 SEK - 1126.00 SEK

Specifications

Antigen CD140b (PDGFRB)
Clone APB5
Concentration 0.5 mg/mL
Applications Flow Cytometry, Immunohistochemistry (Frozen)
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15247227
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Affymetrix eBioscience
14-1402-81
50 μg
550.00 SEK
50µg
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15257227
View Documents Promotion Details
Affymetrix eBioscience
14-1402-82
100 μg
1126.00 SEK
100µg
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Description

Description

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions. Applications Reported: The APB5 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified APB5, cat. 16-1402, in functional assays.). Applications Tested: The APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.

PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
Specifications

Specifications

CD140b (PDGFRB)
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
AI528809; Beta platelet-derived growth factor receptor; beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140B; CD140b antigen; IBGC4; IMF1; JTK12; KOGS; PDGF beta chain; PDGF Receptor beta; Pdgfr; Pdgfr1; PDGFR-1; PDGFRB; PDGF-R-beta; PDGFR-beta; PENTT; platelet derived growth factor receptor beta; platelet derived growth factor receptor, beta polypeptide; platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1; Platelet-derived growth factor receptor, beta; platelet-derived growth factor receptor, beta polypeptide
Pdgfrb
Primary
4° C
Pdgfrb
APB5
Flow Cytometry, Immunohistochemistry (Frozen)
Unconjugated
Rat
Mouse
P05622
18596
IgG2a κ
Affinity chromatography
Antibody
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