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CD142, Functional Grade, clone: HTF-1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-1429-85

Additional Details : Weight : 0.09500kg

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Product Code. 15517786

  • 2970.00 SEK / 500µg


EU Stock 1
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Description

Description

Description: This HTF-1 monoclonal antibody reacts with human CD142, which is also known as Tissue Factor. Expression of this type I transmembrane glycoprotein on endothelial cells, monocytes, macrophages, and platelets can be induced by inflammatory mediators (e.g., LPS, IL-1b, TNFa, PMA, or endotoxin). On the other hand, CD142 is expressed constitutively by some tumor cells (e.g., lung, pancreatic, breast, and colon) and non-immune tissues such as the vasculature, central nervous system, kidney, epithelia, and placenta. Studies have also suggested that CD142 exists as a soluble form that circulates in blood. CD142 initiates blood coagulation by associating with and activating the circulating factors VII and VIIa.The HTF-1 antibody has been reported to exhibit blocking activity.Applications Reported: This HTF-1 antibody has been reported for use in functional assays, flow cytometric analysis, western blotting, and immunocytochemistry.Applications Tested: This HTF-1 antibody has been tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD142
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P13726
F3, Coagulation factor 3, TF, TFA
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow CytometryþFunctional AssayþImmunocytochemistryþWestern Blot
HTF-1
Functional Grade
F3
Purified
F3
IgG1, kappa
500μg
Store at 2-8°C.
Primary
2152
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