CD142, Functional Grade, clone: HTF-1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-1429-85
Additional Details : Weight : 0.09500kg
DescriptionDescription: This HTF-1 monoclonal antibody reacts with human CD142, which is also known as Tissue Factor. Expression of this type I transmembrane glycoprotein on endothelial cells, monocytes, macrophages, and platelets can be induced by inflammatory mediators (e.g., LPS, IL-1b, TNFa, PMA, or endotoxin). On the other hand, CD142 is expressed constitutively by some tumor cells (e.g., lung, pancreatic, breast, and colon) and non-immune tissues such as the vasculature, central nervous system, kidney, epithelia, and placenta. Studies have also suggested that CD142 exists as a soluble form that circulates in blood. CD142 initiates blood coagulation by associating with and activating the circulating factors VII and VIIa.The HTF-1 antibody has been reported to exhibit blocking activity.Applications Reported: This HTF-1 antibody has been reported for use in functional assays, flow cytometric analysis, western blotting, and immunocytochemistry.Applications Tested: This HTF-1 antibody has been tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|F3, Coagulation factor 3, TF, TFA|
|Flow CytometryþFunctional AssayþImmunocytochemistryþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.