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CD150 Monoclonal Antibody (9D1), FITC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
3090.00 SEK
Specifications
Antigen | CD150 |
---|---|
Clone | 9D1 |
Concentration | 0.5 mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Description
Description: The 9D1 monoclonal antibody reacts with mouse CD150, an ∽70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody. Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers, such as CD150+CD244-CD48-. For this application we recommend the use of antibody clone mShad150 (cat. 12-1502). Applications Reported: This 9D1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells. Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).Specifications
CD150 | |
0.5 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
Q9QUM4 | |
Slamf1 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Slamf1 |
9D1 | |
Flow Cytometry | |
FITC | |
Rat | |
Mouse | |
27218 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.