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CD152 (CTLA-4), PE, clone: UC10-4B9, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-1522-83

Additional Details : Weight : 0.25000kg

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Product Code. 15268689

  • 2535.00 SEK / 200µg


EU Stock 1
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Description

Description

Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly. Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Applications Reported: This UC10-4B9 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.Applications Tested: This UC10-4B9 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the FoxP3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD152 (CTLA-4)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P09793
CELIAC3, CTLA-4, GRD4, GSE, ICOS, IDDM12, CD152 isoform, celiac disease 3, cytotoxic T lymphocyte associated antigen 4 short spliced form, cytotoxic T-lymphocyte antigen 4
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Flow Cytometry
UC10-4B9
PE
Ctla4
Liquid
Ctla4
IgG
200μg
4° C, store in dark, DO NOT FREEZE!
Primary
12477
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