CD152 (CTLA-4), PE, clone: eBio20A (20A, A3), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-1528-42
Additional Details : Weight : 0.23750kg
DescriptionDescription: The eBio20A monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells (activated B cells may also express CTLA-4) and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling.Applications Reported: This eBio20A (20A, A3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio20A (20A, A3) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of PHA stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CELIAC3, CTLA-4, GRD4, GSE, ICOS, IDDM12, CD152 isoform, celiac disease 3, cytotoxic T lymphocyte associated antigen 4 short spliced form, cytotoxic T-lymphocyte antigen 4|
|eBio20A (20A, A3)|
|IgG2a, kappa, kappa|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.