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CD16/CD32 Monoclonal Antibody (93), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

642.00 SEK - 4330.00 SEK

Specifications

Antigen CD16/CD32
Clone 93
Concentration 0.5 mg/mL
Applications Flow Cytometry, Functional Assay, Neutralization
Classification Monoclonal
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Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15226827
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Affymetrix eBioscience
14-0161-81
50 μg
642.00 SEK
50µg
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15236827
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Affymetrix eBioscience
14-0161-82
100 μg
990.00 SEK
100µg
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15246827
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Affymetrix eBioscience
14-0161-85
500 μg
2420.00 SEK
500µg
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15243318
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Affymetrix eBioscience
14-0161-86
1 mg
4330.00 SEK
1mg
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Description

Description

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils. Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis. 93 has also been reported in blocking of Fc-mediated reactions in functional studies. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with 0.5-1 μg of purified anti-CD16/CD32 per million cells for 5-10 minutes on ice prior to staining. (Please use Functional Grade purified 93, cat. 16-0161, in functional assays.). Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.
Specifications

Specifications

CD16/CD32
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
AI528646; CD16; CD16a; CD16a antigen; CD16B; CD32; CD32 receptor 2; CD32B; CDw32; F630109E10Rik; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIb; Fc fragment of IgG receptor IIIa; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); fc gamma receptor IIB; Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor RII; Fc gamma RIIB; Fc gamma RIIIa; Fc receptor, IgG, low affinity IIb; Fc receptor, IgG, low affinity III; Fc[g]RII; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); fc-gamma RII; Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; Fc-gamma-RII; FcgammaRIIB; fc-gamma-RIIB; Fc-gamma-RIIc; FcgammaRIII; FcgammaRIIIA; Fcgr2; Fcgr2a; Fcgr2b; FCGR2C; Fcgr3; FCGR3A; FCGR3B; FcgRII; FCGRIII; FCR-10; Fcr-2; Fcr-3; fcRII; FcRII-b; Fc
Fcgr2b, Fcgr3
Primary
4° C
93
Flow Cytometry, Functional Assay, Neutralization
Unconjugated
Rat
Mouse
P08101, P08508
14130, 14131
IgG2a λ
Affinity chromatography
Antibody
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