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CD16, Biotin, clone: eBioCB16 (CB16), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-0168-80

Additional Details : Weight : 0.25000kg

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Product Code. 15269469

  • 764.00 SEK / 25µg


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Description

Description

Description: The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.Applications Reported: This eBioCB16 (CB16) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioCB16 (CB16) antibody has been tested by flow cytometric analysis of normal normal human peripheral blood cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD16
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O75015, P08637
FCGR3A, FCGR3B, CD16A, CD16B, Fc gamma Receptor III, Fc gamma Receptor 3, RP11-5K23.1, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
eBioCB16 (CB16)
Biotin
FCGR3A
Liquid
FCGR3A, FCGR3B
IgG1, kappa, kappa
25μg
Store at 2-8°C. Do not freeze.
Primary
2214, 2215
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