CD161, PE-eFluor 610, clone: HP-3G10, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 61-1619-42
Additional Details : Weight : 0.23750kg
DescriptionDescription: This HP-3G10 monoclonal antibody reacts with CD161 (also known as NKR-P1A), a member of the C-type lectin superfamily. The human homologue of NK1.1 in C57BL/6 mice, CD161 is expressed on natural killer cells and is upregulated in response to IL-12. CD161 can also be detected on various T cell subsets, including memory/effector CD4+ T cells, CD8+ T cells, gamma delta TCR T cells, and a subset of CD3+ thymocytes. Finally, CD161 expression has been demonstrated on human Th17 CD4+ T cells. The function of this receptor is unclear although studies suggest a possible stimulatory role. Nevertheless, Lectin-like transcript-1 (LLT1), which is also known as osteoclast inhibitory lectin, has been identified as the ligand for CD161.Applications Reported: This HP-3G10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HP-3G10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PE-eFluor™ 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red™). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Ly55a, NKR-P1A, NKRP12, Nkrp1-a, Nkrp1a|
|IgG1, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.|
For Research Use Only.