CD182 (CXCR2), clone: eBio5E8-C7-F10 (5E8-C7-F10), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1829-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8Rbeta). CD182 is a 67-70kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8Ralpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.Applications Reported: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CXCR2, CDw128b, CD182 antigen, CMKAR2, CXCR-2, CD182, CXC-R2, IL-8R B, Il-8 receptor b, IL-8 receptor type 2, Il-8rb, IL8R2, IL8RB, IL-8 receptor beta|
|IgG1, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.