missing translation for 'onlineSavingsMsg'
Learn More

CD184 (CXCR4), clone: 12G5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9999-82

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15258327

  • 1520.00 SEK / 100µg


Estimated Shipment date
from Supplier 31-10-2018
Add to basket

Description

Description

Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.Applications Reported: The 12G5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CXCR4 in functional studies. (Please use Functional Grade purified 12G5, cat. 16-9999, in functional assays.).Applications Tested: The 12G5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD184 (CXCR4)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P61073
CXC Chemokine Receptor 4, Fusin, LESTR, HUMSTR
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry, Functional Assay, Neutralization
12G5
Unconjugated
CXCR4
Liquid
CXCR4
IgG2a, kappa, kappa
100μg
Store at 2-8°C.
Primary
7852
Videos

For Research Use Only.