CD19, clone: LC1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0190-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: The LC1 monoclonal antibody reacts with human CD19, a 95kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multi-molecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The antibody recognizes a sequence near the carboxy terminus.Applications Reported: This LC1 antibody has been reported for use in western blotting and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This LC1 antibody has been tested by western blot and can be used at less than or equal to 5 μg/mL. This antibody has also been tested by immunohistochemistry on FFPE human tonsil using high pH antigen retrieval at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|AW495831, B-lymphocyte antigen CD19, differentiation antigen CD19|
|Immunohistochemistry (Paraffin), Western Blot|
|IgG1, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.