CD201 (EPCR), Biotin, clone: eBio1560 (1560), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 13-2012-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: The eBio1560 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25kDa Type 1 transmembrane protein expressed on endothelial cells. EPCR exhibits sequence and structural homology with the MHC class I/CD1 family of proteins. EPCR is a ligand for Protein C and plays an important role in augmenting Protein C activation by the thrombin-thrombomodulin complex and in regulating blood coagulation and inflammation. Deletion of EPCR results in embryonic lethality, at least partically due to placental thrombosis. Recently, it was demonstrated that EPCR expression identified cells in the bone marrow that are capable of hematopoietic reconstitution activity comparable to hematopoietic stem cells isolated with conventional methods. The eBio1560 monoclonal antibody can be used for the detection of these hematopoietic stem cells, however the eBiomRCR-16 monoclonal antibody should only be used for the detection of CD201 on endothelial cells.Applications Reported: This eBio1560 (1560) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.Applications Tested: This eBio1560 (1560) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest, particμLarly when analyzing expression on endothelial cells where CD201 expression is very high.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Human Endothelial Cell Protein C Receptor|
|IgG2b, kappa, kappa|
|Store at 2-8°C. Do not freeze.|
For Research Use Only.