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CD203c, PE-Cyanine7, clone: NP4D6, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-2039-41

Additional Details : Weight : 0.23750kg

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Product Code. 15518516

  • 1218.00 SEK / 25 tests


Estimated Shipment date
from Supplier 25-07-2018
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Description

Description

Description: This NP4D6 monoclonal antibody reacts with human CD203c, which is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3). ENPP family members catalyze the hydrolysis of pyrophosphate and phosphodiester bonds to nucleoside 5-monophosphates. A type II transmembrane protein, CD203c is expressed on basophils and mast cells. CD203c expression is upregulated on basophils activated by allergen or IgE crosslinking. Therefore, CD203c is used as an indicator of basophil activation in various IgE-mediated allergic responses.Applications Reported: This NP4D6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This NP4D6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD203c
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O14638
B10, CD203c, NPP3, PD-IBETA, PDNP3, E-NPP 3
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
NP4D6
PE-Cyanine7
ENPP3
Conjugated
ENPP3
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
5169
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