CD207 (Langerin), clone: eBioL31, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-2075-80
Additional Details : Weight : 0.01000kg
DescriptionApplications Reported: This eBioL31 antibody has been reported for use in flow cytometric analysis, intracellular staining followed by flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. (Fluorochrome conjugated eBioL31 is recommended for use in intracellular flow cytometry.). Applications Tested: This eBioL31 antibody has been tested by flow cytometric analysis of transfected cells and western blotting of mouse ear lysates. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Lamgerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.
|PBS with 0.09% sodium azide; pH 7.2|
|Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot|
For Research Use Only.