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CD207 (Langerin), clone: eBioL31, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-2075-82

Additional Details : Weight : 0.01000kg

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Product Code. 15257307

  • 2120.00 SEK / 100µg


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Description

Description

Description: The eBioL31 monoclonal antibody reacts with mouse CD207, also known as Langerin, which is expressed in a distinct subset of dendritic cells called Langerhans cells (LC). Mouse CD207 is a 48kDa C-type lectin transmembrane protein that likely plays a role in antigen recognition and uptake. LC are located in the epidermis and upon activation, reduce CD207 expression and begin migrating through the dermis towards lymphatic vessels. Expression of mouse CD207 in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of CD207 cDNA into fibroblast cell lines.Western Blotting with eBioL31 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse CD207 cDNA. Preliminary data from mouse CD207 cDNA transfected cells suggests that eBioL31 can be used for intracellular flow cytometric analysis in combination with the Foxp3/Transcription Factor Staining Bufer Set (cat. 00-5523). The epitope recognized by eBioL31 is in the extracellular domain within the CRD (carbohydrate recognition domain) and therefore can be used to detect both surface and intracellular langerin. Differences in expression has been observed between mouse strains.Applications Reported: This eBioL31 antibody has been reported for use in flow cytometric analysis, intracellular staining followed by flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. (Fluorochrome conjugated eBioL31 is recommended for use in intracellular flow cytometry.).Applications Tested: This eBioL31 antibody has been tested by flow cytometric analysis of transfected cells and western blotting of mouse ear lysates. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD207 (Langerin)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q8VBX4
CD207
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot, Western Blot
eBioL31
Unconjugated
Cd207
Liquid
Cd207
IgG2a
100μg
Store at 2-8°C.
Primary
246278
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