CD209 (DC-SIGN), clone: eB-h209, eBioscience™

Description

Description: The eB-h209 monoclonal antibody reacts with human CD209, also known as DC-SIGN, a 44kDa type II transmembrane protein. DC-SIGN contains a C-type lectin binding domain and binds ICAM-3, ICAM-2, and HIV virus. Human dendritic cells preferentially express DC-SIGN. It has been postulated that DC-SIGN serves as a receptor for capture, trafficking, and transmission of HIV to T cells and supports primary immune response. eB-h209 was developed against a C-terminal peptide of human DC-SIGN.Applications Reported: The eB-h209 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.Applications Tested: The eB-h209 antibody has been tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.