CD221 (IGF1R), Alexa Fluor 488, clone: 1H7, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 53-8849-42
Additional Details : Weight : 0.23750kg
DescriptionDescription: This 1H7 monoclonal antibody reacts with human insulin-like growth factor-1 receptor (IGF-1R), also known as CD221. This receptor is a cell surface-expressed glycoprotein composed of two extracellular alpha subunits and two transmembrane beta subunits that possess tyrosine kinase activity. Expressed on nearly all cell types, this receptor binds IGF-I and IGF-II, as well as insulin. Ligand binding leads to activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival. Many tumors and transformed cells display altered IGF-1R expression.This monoclonal antibody has been reported to block binding of IGF-I and IGF-II to the IGF-1R.Applications Reported: This 1H7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1H7 antibody has been pre-titrated and tested by flow cytometric analysis of the HeLa cell line. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Alexa Fluor 488|
|IgG1, kappa, kappa|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.