missing translation for 'onlineSavingsMsg'
Learn More

CD223 (LAG-3) Mouse anti-Human, Super Bright 645, Clone: 3DS223H, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 64-2239-42

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15933644

  • 3570.00 SEK / Pack of 100
Estimated Shipment: 14-12-2022
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predomitly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969). Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometic analysis of PHA stimulated human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4 degrees C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).
Specifications

Specifications

CD223 (LAG-3)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P18627
LAG3
Affinity chromatography
RUO
3902
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
3DS223H
Super Bright 645
LAG3
CD223; LAG-3; Ly66; Lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC
Mouse
100 Tests
Primary
Human
Antibody
IgG1 κ
Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers