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CD24 Rat anti-Mouse, Biotin, Clone: M1/69, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-0242-82

Additional Details : Weight : 0.25000kg

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Product Code. 15219499

  • 1380.00 SEK / 100µg

Estimated Shipment: 28-07-2021

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Description

Description

Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24. Applications Reported: The M1/69 antibody has been reported for use in flow cytometric analysis. Applications Tested: The M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin′s lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.
Specifications

Specifications

CD24
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P24807
CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24A; FLJ22950; FLJ43543; MGC75043
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
M1/69
Biotin
Cd24a
Liquid
Cd24a
IgG2b, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12484
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For Research Use Only.