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CD24 Monoclonal Antibody (30-F1), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

1112.00 SEK - 2440.00 SEK

Specifications

Antigen CD24
Clone 30-F1
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15248489
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Affymetrix eBioscience
12-0241-82
100 μg
1112.00 SEK
100µg
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15258489
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Affymetrix eBioscience
12-0241-83
200 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The 30-F1 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. The expression of CD24 detected by 30-F1 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and different antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. Applications Reported: The 30-F1 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 30-F1 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.
Specifications

Specifications

CD24
0.2 mg/mL
Monoclonal
Liquid
RUO
P24807
Cd24a
Primary
4° C, store in dark, DO NOT FREEZE!
Cd24a
30-F1
Flow Cytometry
PE
Rat
Mouse
12484
IgG2c κ
Affinity chromatography
Antibody
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