missing translation for 'onlineSavingsMsg'
Learn More

CD25 Monoclonal Antibody (BC96), PE-Cyanine5, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

1368.00 SEK - 3030.00 SEK

Specifications

Antigen CD25
Clone BC96
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
View More Specs

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15527726
View Documents Promotion Details
Affymetrix eBioscience
15-0259-42
100 Tests
3030.00 SEK
100 tests
View alternative products
Save Up to 
»
In Stock
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15517726
View Documents Promotion Details
Affymetrix eBioscience
15-0259-41
25 Tests This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A View alternative products
Save Up to 
»
Description

Description

Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to μgcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Specifications

Specifications

CD25
5 μL/Test
Monoclonal
Liquid
RUO
P01589
IL2RA
Primary
4° C, store in dark, DO NOT FREEZE!
IL2RA
BC96
Flow Cytometry
PE-Cyanine5
Mouse
Human
3559
IgG1 κ
Affinity chromatography
Antibody
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.