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CD253 (TRAIL) Monoclonal Antibody (N2B2), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-5951-050
Beskrivning
Description: The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3^- NK1.1^+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells. Applications Reported: N2B2 has been reported for use in flow cytometric analysis, and blocking of TRAIL in functional assays. Applications Tested: The N2B2 antibody has been tested by flow cytometric analysis of mouse TRAIL transfected cells and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
CD253 (TRAIL) Monoclonal antibody specifically detects CD253 (TRAIL) in Mouse samples. It is validated for Flow Cytometry, Functional Assay, Neutralization
Specifikationer
| CD253 (TRAIL) | |
| Monoclonal | |
| 1 mg/mL | |
| PBS with no preservative; pH 7.2 | |
| P50592 | |
| Rat | |
| 50 mg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2a κ |
| Flow Cytometry, Functional Assay, Neutralization | |
| N2B2 | |
| Functional Grade | |
| TNFSF10 | |
| A330042I21Rik; AI448571; Apo-2 ligand; APO2L; Apo-2L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Ly81; Protein TRAIL; TL2; TN; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; TNFSF10; TNLG6A; Trail; TRAIL protein; TRAIL/APO2L; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; tumor necrosis factor ligand superfamily member 10; tumor necrosis factor superfamily member 10 | |
| Affinity chromatography | |
| RUO | |
| 22035 | |
| 4° C | |
| Liquid |
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