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CD265 (RANK) Rat anti-Mouse, Clone: R12-31, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-6612-85
Additional Details : Weight : 0.01000kg
Description
Description: The R12-31 monoclonal antibody reacts with mouse RANK (Receptor activator of NF-kappaB), a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kappaB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kappaB, whereas JNK activation is mediated through binding of RANK to TRAF2. Applications Reported: The R12-31 antibody has been reported for use in flow cytometric analysis. Applications Tested: The R12-31 antibody has been tested by flow cytometric analysis of mouse RANK transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full length mouse RANK cDNA encodes a type I transmembrane protein with an extracellular and a cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNFR family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains.Specifications
CD265 (RANK) | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
O35305 | |
Ly109, ODFR, OFE, Rank, TRANCE-R, mRANK | |
Rat | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Mouse |
Flow Cytometry | |
R12-31 | |
Unconjugated | |
Tnfrsf11a | |
Liquid | |
Tnfrsf11a | |
IgG2a, kappa | |
500 μg | |
4° C | |
Primary | |
21934 |
For Research Use Only.