CD273 (B7-DC), clone: TY25, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5986-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The TY25 monoclonal antibody reacts with mouse B7-DC/PD-L2. B7-DC, a member of the B7 family, has a predicted molecular weight of ∽25kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by sub-populations of dendritic cells, monocytes and macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, but binds PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. TY25 is a useful tool to study the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in mouse.Applications Reported: The TY25 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. Immunoblotting should be carried out under non-reducing conditions. It has also been reported for inhibition of ligand binding. (Please use Functional Grade purified TY25, cat. 16-5986, in functional assays.).Applications Tested: The TY25 antibody has been tested by flow cytometric analysis of mouse splenocytes and B7-DC transfected cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|B7-DC, Btdc, F730015O22Rik, MGC124039, MGC124040, PD-L2|
|Flow CytometryþFunctional AssayþImmunohistochemistry (Frozen)þImmunoprecipitationþNeutralizationþWestern Blot|
|IgG2a, kappa, kappa|
For Research Use Only.