CD275 (B7-H2), Biotin, clone: MIH12, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 13-5889-82
Additional Details : Weight : 0.25000kg
DescriptionDescription: The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.Applications Reported: This MIH12 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. Applicability of MIH12 for immunoprecipitation and immunoblotting (WB) has not been determined.Applications Tested: The MIH12 antibody has been tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, KIAA0653, LICOS, ICOS Ligand|
|Flow CytometryþImmunohistochemistry (Frozen)|
|Store at 2-8°C. Do not freeze.|
For Research Use Only.