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CD28 Syrian Hamster anti-Mouse, Clone: 37.51, eBioscience™

Syrian Hamster Monoclonal Antibody

430.00 SEK - 3730.00 SEK

Specifications

Antigen CD28
Clone 37.51
Target Species Mouse
Conjugate Unconjugated
Isotype IgG
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Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15296877
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Affymetrix eBioscience
14-0281-81
50 μg
430.00 SEK
50µg
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15206887
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Affymetrix eBioscience
14-0281-82
100 μg
862.00 SEK
100µg
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15216887
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Affymetrix eBioscience
14-0281-85
500 μg
1954.00 SEK
500µg
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15226887
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Affymetrix eBioscience
14-0281-86
1 mg
3730.00 SEK
1mg
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Description

Description

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 37.51 has also been reported in costimulation of T cells in vitro and in vivo . (Please use Functional Grade purified 37.51, cat. 16-0281, in functional assays.). Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary’s Disease.
Specifications

Specifications

CD28
Mouse
IgG
Monoclonal
Liquid
Antibody
4° C
37.51
Unconjugated
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.