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CD28 Monoclonal Antibody (CD28.2), PerCP-Cyanine5.5, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
1540.00 SEK - 3320.00 SEK
Specifications
Antigen | CD28 |
---|---|
Clone | CD28.2 |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15559016
|
Affymetrix eBioscience
45-0289-42 |
100 Tests |
3320.00 SEK
100 tests |
Estimated Shipment: 10-05-2024 Log in to see stock. |
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15549016
|
Affymetrix eBioscience
45-0289-41 |
25 Tests |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. Applications Reported: This CD28.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CD28.2 antibody has been pre-titrated and tested by flow cytometric analysi sof normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.Specifications
CD28 | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
MGC138290, Tp44, CD28 antigen, T-cell-specific surface glycoprotein CD28 | |
CD28 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
CD28 |
CD28.2 | |
Flow Cytometry | |
PerCP-Cyanine5.5 | |
Mouse | |
Human | |
P10747 | |
940 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.