CD28, clone: CD28.2, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0289-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. The recommended titer for immunoprecipitation is ∽2-5 μg per million cells. CD28.2 has also been reported in costimulation of T cells in in vitro functional assays. (Please use Functional Grade purified CD28.2, cat. 16-0289, in functional assays.).Applications Tested: The CD28.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|MGC138290, Tp44, CD28 antigen, T-cell-specific surface glycoprotein CD28|
|Flow CytometryþFunctional AssayþImmunohistochemistryþImmunoprecipitation|
|IgG1, kappa, kappa|
For Research Use Only.