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CD28 Monoclonal Antibody (JJ319), Functional Grade, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
1110.00 SEK - 3555.00 SEK
Specifications
Antigen | CD28 |
---|---|
Clone | JJ319 |
Concentration | 1 mg/mL |
Applications | Flow Cytometry, Functional Assay |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15298407
|
Affymetrix eBioscience
16-0280-81 |
50 μg |
1110.00 SEK
50µg |
Estimated Shipment: 10-04-2024 Log in to see stock. |
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15208417
|
Affymetrix eBioscience
16-0280-85 |
500 μg |
3555.00 SEK
500µg |
In Stock Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
Description: The JJ319 monoclonal antibody reacts with the rat CD28 molecule, expressed by thymocytes, mature T cells, and a subset of NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. No reactivity of JJ319 is observed on mouse T cells. Applications Reported: The JJ319 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro T cell costimulation. Applications Tested: This JJ319 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.Specifications
CD28 | |
1 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with no preservative; pH 7.2 | |
antigen CD28; CD28; CD28 antigen; CD28 antigen (Tp44); CD28 isoform; CD28 isoform 2; Cd28 molecule; CD28 precursor protein; CD28 protein; CD28 protein precursor; CD28RNA; cell surface protein; CHT28; contains partial extracellular domain; costimulatory molecule B7 receptor CD28; EGK_04709; MGC138290; sCD28; soluble CD28; T44; T-cell costimulatory molecule CD28; T-cell costimulatory molecule Tp44; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28 homolog; TP44 | |
Cd28 | |
Primary | |
4° C | |
Cd28 |
JJ319 | |
Flow Cytometry, Functional Assay | |
Functional Grade | |
Mouse | |
Rat | |
P31042 | |
25660 | |
IgG1 | |
Affinity chromatography | |
Antibody |
For Research Use Only.