CD282 (TLR2), Functional Grade, clone: TL2.1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-9922-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (∽90 kDa) from PBMC and HMEC.Applications Reported: TL2.1 has been reported for use in flow cytometric analysis and blocking of TLR2-mediated cytokine production. Inhibition of cytokine production has been reported in the literature using Listeria monocytogenes and peptidoglycans. Optimal conditions for the inhibition assays using TL2.1 and specific stimulatory reagents/suppliers have to be evaluated by individual investigators. Neutralization Protocol: (Important note: make sure to eliminate LPS contamination in the culture system) 1) Prepare target cells of interest at the concentration of 2 x 10^6 /mL in 10% FBS-IMDM (or 5% human serum). 2) Pre-incubate the cells with 10-20 μg/mL of TL2.1 or isotype control antibody at room temperature for 30min. 3) Add the stimuli and incubate at 37°C, 5% CO2 for 14 hours. Harvest the supernatants. 4) Follow the protocol of eBioscience Ready-SET-Go ELISA set to detect IL-6 or TNF alpha production.Applications Tested: The TL2.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|TIL4, Toll-like Receptor 2, toll/interleukin 1 receptor-like 4, toll/interleukin-1 receptor-like protein 4|
|Flow CytometryþFunctional AssayþNeutralization|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.