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CD284 (TLR4) Mouse anti-Human, Clone: HTA125, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9917-80

Additional Details : Weight : 0.01000kg

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Product Code. 15248237

  • 864.00 SEK / 25µg


Estimated Shipment date
from Supplier 31-07-2019
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Description

Description

For detection of peripheral monocytes, a three step staining protocol is recommended using purified anti-human TLR4 followed by biotin anti- mouse IgG and streptavidin-PE. (Please use Functional Grade purified HTA125, cat. 16-9917, in functional assays.). Applications Tested: The HTA125 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. It is highly recommended that for optimal staining of TLR4, whole blood be stained using the LWB protocol found in Best Protocols rather than Ficoll-gradient prepared normal human peripheral blood cells. The use of a density gradient appears to reduce the staining intensity significantly. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. TLR4 is a member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.
Specifications

Specifications

CD284 (TLR4)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
O00206
Toll-like Receptor 4, ARMD10, CD284, TLR-4, TOLL, hToll, homolog of Drosophila toll
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization
HTA125
Unconjugated
TLR4
Liquid
TLR4
IgG2a, kappa
25 μg
4° C
Primary
7099
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