CD284 (TLR4), clone: HTA125, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9917-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (∽100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors.Applications Reported: The HTA125 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported in blocking of LPS-induced cytokine production. For detection of peripheral monocytes, a three step staining protocol is recommended using purified anti-human TLR4 followed by biotin anti-mouse IgG and streptavidin-PE. (Please use Functional Grade purified HTA125, cat. 16-9917, in functional assays.).Applications Tested: The HTA125 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.It is highly recommended that for optimal staining of TLR4, whole blood be stained using the LWB protocol found in Best Protocols rather than Ficoll-gradient prepared normal human peripheral blood cells. The use of a density gradient appears to reduce the staining intensity significantly.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Toll-like Receptor 4, ARMD10, CD284, TLR-4, TOLL, hToll, homolog of Drosophila toll|
|Flow CytometryþFunctional AssayþImmunohistochemistryþImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.