CD284 (TLR4), clone: HTA125, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9917-82
Additional Details : Weight : 0.01000kg
DescriptionFor detection of peripheral monocytes, a three step staining protocol is recommended using purified anti-human TLR4 followed by biotin anti- mouse IgG and streptavidin-PE. (Please use Functional Grade purified HTA125, cat. 16-9917, in functional assays.). Applications Tested: The HTA125 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. It is highly recommended that for optimal staining of TLR4, whole blood be stained using the LWB protocol found in Best Protocols rather than Ficoll-gradient prepared normal human peripheral blood cells. The use of a density gradient appears to reduce the staining intensity significantly. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. TLR4 is a member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.
|PBS with 0.09% sodium azide; pH 7.2|
|Toll-like Receptor 4, ARMD10, CD284, TLR-4, TOLL, hToll, homolog of Drosophila toll|
|Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization|
For Research Use Only.