CD289 (TLR9), clone: eB72-1665, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9099-80
Additional Details : Weight : 0.01000kg
DescriptionHuman Epithelial Cell lines were also reported to stain with this mAb (J. Immunol. 173: 1219). Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry. Applications Reported: This eB72-1665 antibody has been reported for use in intracellular flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). (Fluorochrome conjugated eB72-1665 is recommended for use in intracellular flow cytometry.) Preliminary in vitro blocking assays suggested that eB72-1665 may not be effective in blocking of CpG binding. Please contact us for further information. Applications Tested: The eB72-1665 antibody has been tested by intracellular flow cytometric analysis of hTLR9 transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
|PBS with 0.09% sodium azide; pH 7.2|
|Toll-like Receptor 9; Toll-like receptor 9 protein; UNQ5798/PRO19605|
|Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
For Research Use Only.