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IBA Lifesciences™ CD3/CD28 Streptamer™ Kit, Mouse

Produktkod. 17540655 Handla allt IBA Lifesciences Produkter
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Produktkod. 17540655 Leverantör IBA Lifesciences™ Leverantörsnummer 68920050

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Beskrivning

CD3/CD28 Kit for mouse T cell activation and expansion with removable stimulation reagents for murine samples for 5 x 107 total cells.

The CD3/CD28 Streptamer™ approach is based on our Strep-tag™ technology and facilitates T cell activation and expansion. Low affinity, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) against CD3 and CD28 are multimerized on a Strep-Tactin™ backbone. The resulting CD3/CD28 Streptamer™ complex stimulates the T cell receptor/CD3 complex (TCR/CD3; signal 1) and the co-stimulatory molecule CD28 (signal 2). Providing these two signals leads to efficient T cell activation and proliferation.

For in vitro stimulation and activation, the soluble CD3/CD28 Streptamers are added to T cells. The addition of biotin reverses the multimerization and causes the dissociation of low affinity Fab fragments from the cells. Consequently, the termination of stimulation is possible at any point in time.

  • Reversible reagents due to low affinity Fab fragments
  • Accurately defined termination of stimulation

Specifikationer

Celltyp Primary Murine T Cells
Målarter Murine
För användning med (applikation) T Cell Activation and Expansion
Tillräckligt för 5 x 107 Total Cells
Kvantitet 1 Kit
Produkttitel
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