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CD301b (MGL2) Monoclonal Antibody (11A10-B7), eFluor™ 660, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 50-3011-82
Additional Details : Weight : 0.09500kg
Description
Description: The monoclonal antibody 11A10-B7 recognizes mouse CD301b. CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming. Applications Reported: This 11A10-B7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 11A10-B7 antibody has been tested by flow cytometric analysis of mouse skin draining lymph node cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm).
CD301b (MGL2) Monoclonal antibody specifically detects CD301b (MGL2) in Mouse samples. It is validated for Flow Cytometry.Specifications
| CD301b (MGL2) | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| 0 | |
| Mgl2 | |
| Affinity chromatography | |
| RUO | |
| 216864 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| 11A10-B7 | |
| eFluor 660 | |
| Mgl2 | |
| CD301; CD301b; macrophage galactose N-acetyl-galactosamine specific lectin 2; macrophage galactose-type C-type lectin 2; Mgl2 | |
| Rat | |
| 100 μg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2a λ |
For Research Use Only.