missing translation for 'onlineSavingsMsg'
Learn More

CD325 (N-Cadherin) Mouse anti-Human, Biotin, Clone: 8C11, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-3259-80

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15239789

  • 1016.00 SEK / 25µg

Estimated Shipment: 27-07-2021

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The 8C11 monoclonal antibody reacts with human CD325, also known as N-Cadherin. CD325 is a 130 kDa member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently dysregulated in cancer cells. Applications Reported: This 8C11 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 8C11 antibody has been tested by flow cytometric analysis of the HeLa cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin′s function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
Specifications

Specifications

CD325 (N-cadherin)
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P19022
Cadherin-2, Neural-cadherin, CDw325 antigen, CD325, CDHN, NCAD, Cadherin-N
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry, Immunohistochemistry (Paraffin)
8C11
Biotin
CDH2
Liquid
CDH2
IgG1, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
1000
Videos
SDS
Documents
Certificates

For Research Use Only.