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CD326 (EpCAM), eFluor 660, clone: 1B7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9326-42

Additional Details : Weight : 0.23750kg

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Product Code. 15520097

  • 2240.00 SEK / 100 tests


EU Stock 9
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Description

Description

Description: EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions. EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM.Applications Reported: This 1B7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1B7 antibody has been pre-titrated and tested by flow cytometric analysis of human A549 cell line. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD326
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P16422
Epithelial Specific Antigen, ESA, KSA, M4S1, MK-1, DIAR5, EGP-2, EGP40, KS1/4, MIC18, TROP1, TACSTD1, EPCAM, Epithelial cell adhesion molecule
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
1B7
eFluor 660
EPCAM
Conjugated
EPCAM
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
4072
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