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CD326 (EpCAM) Rat anti-Mouse, Super Bright 600, Clone: G8.8, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 63-5791-82

Additional Details : Weight : 0.01000kg

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Product Code. 15933554

  • 3230.00 SEK / 100µg
Estimated Shipment: 19-12-2022
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Description

Description

Description: The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. The antigen is being used as a target for immunotherapy treatment of human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation. This epithelial glycoprotein is now recognized as having an important role in tumor biology. Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of TE-71 cell line. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4 degrees C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. Ep-CAM is a ∽40 kD glycoprotein, which functions as an epithelial cell adhesion molecule. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is expressed in the majority of human carcinomas and is a marker for tumors of epithelial lineage.
Specifications

Specifications

CD326 (EpCAM)
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
Q99JW5
Epcam
Affinity chromatography
RUO
17075
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
G8.8
Super Bright 600
Epcam
CD326; DIAR5; EGP-2; EGP314; EGP40; Ep-CAM; EPCAM; Epithelial cell adhesion molecule; Epithelial glycoprotein 314; Epithelial Specific Antigen; KS1/4; lymphocyte antigen 74; M4S1; mEGP314; MIC18; MK-1; panepithelial glycoprotein 314; Protein 289A; TACSTD1; Trop-1 protein; TROP1; Tumor-associated calcium signal transducer 1
Rat
100 μg
Primary
Mouse
Antibody
IgG2a κ
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