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CD33, clone: WM-53 (WM53), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0338-82

Additional Details : Weight : 0.01000kg

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Product Code. 15266907

  • 932.00 SEK / 100µg


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.Applications Reported: The WM-53 (WM53) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunocytochemistry staining.Applications Tested: The WM-53 (WM53) antibody has been tested by flow cytometric analysis of lysed whole blood. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD33
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P20138
FLJ00391, SIGLEC-3, p67
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow CytometryþImmunocytochemistryþImmunoprecipitation
WM-53 (WM53)
Unconjugated
CD33
Purified
CD33
IgG1, kappa
100μg
Store at 2-8°C.
Primary
945
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