CD336 (NKp44), eFluor 450, clone: 44.189, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-3369-41
Additional Details : Weight : 0.23750kg
DescriptionDescription: The monoclonal antibody 44.189 reacts with human CD336, which is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.The 44.189 antibody has been reported to facilitate CD336 crosslinking.Applications Reported: This 44.189 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 44.189 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (1.0 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CD336, LY95, NCR2, CD336, CD336 antigen, LY95lymphocyte antigen 95 homolog (activating NK-receptor, NK-p44), lymphocyte antigen 95 (activating NK-receptor, NK-p44), Lymphocyte antigen 95 homolog, natural cytotoxicity triggering receptor 2, Natural killer cell p44-related protein, NK cell activating receptor (NKp44), NK cell-activating receptor, NKp44, NKP44, NK-p44dJ149M18.1|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.