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CD337 (NKp30), PE-Cyanine7, clone: AF29-4D12, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-3379-42

Additional Details : Weight : 0.23750kg

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Product Code. 15508546

  • 2755.00 SEK / 100 tests


EU Stock 2
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Description

Description

Description: This AF29-4D12 monoclonal antibody reacts with human CD337, which is also known as NKp30. Expressed on NK cells, CD337 is the predominant member of the natural cytotoxicity receptor family that also includes NKp46 and NKp44. CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells. Several ligands for this receptor have been reported, including B7-H6.The AF29-4D12 antibody has been reported to crossreact to rhesus macaque.Applications Reported: This AF29-4D12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF29-4D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD337 (NKp30)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q8MJ02, O14931
1C7, CD337, LY117, MALS, NKp30, NK-p30, activating NK-A1 receptor, activating natural killer receptor p30, lymphocyte antigen 117, natural killer cell p30-related protein
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Non-human Primate, Rhesus Monkey
Flow Cytometry
AF29-4D12
PE-Cyanine7
NCR3
Liquid
NCR3
IgG1, kappa, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
259197, 715574
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