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CD34, FITC, clone: RAM34, eBioscience™

Rat Monoclonal Antibody

782.00 SEK - 2385.00 SEK

Specifications

Antigen CD34
Clone RAM34
Target Species Mouse
Conjugate FITC
Isotype IgG2a, kappa
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15268069 Affymetrix eBioscience
11-0341-85
500 μg 2385.00 SEK
500µg View alternative products
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EU Stock 17
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15248069 Affymetrix eBioscience
11-0341-81
50 μg 782.00 SEK
50µg View alternative products
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EU Stock 7
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15258069 Affymetrix eBioscience
11-0341-82
100 μg 1190.00 SEK
100µg View alternative products
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EU Stock 16
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Description

Description

Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Note: When staining with the RAM34 antibody an incubation time of 90 minutes is recommended to obtain the optimal signal to noise ratio.For more detailed information on staining with RAM34, please refer to Ema et al., listed under References.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD34
Mouse
IgG2a, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
RAM34
FITC
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.