CD368 (Clec6), eFluor 660, clone: 9B9, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-9113-42
Additional Details : Weight : 0.23750kg
DescriptionDescription: This 9B9 monoclonal antibody reacts with human CD368, also known as Clec6 or ClecsF8 or Clec4D. In peripheral blood, this C-type lectin is expressed highly on granulocytes and CD14+CD16- monocytes. Some dendritic cell populations also exhibit low levels of CD368 expression. Studies have demonstrated that this receptor undergoes endocytosis due to its C-type lectin-like domain. CD368 functions as a phagocytic receptor and is involved in proinflammatory cytokine production in cooperation with Toll-like receptors. Finally, CD368 also participates in cellular activation via signaling through Syk kinase.Applications Reported: This 9B9 antibody has been reported for use in flow cytometric analysis. Due to the endocytic nature of Clec6, we recommend fixing your cells prior staining to ensure the maximum possible signal.Applications Tested: This 9B9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|IgG2b, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.